Macromolecular crowding allows blunt-end ligation by DNA ligases from rat liver or Escherichia coli.
In the presence of high concentrations of any of several types of macromolecules, DNA ligase preparations from rat liver nuclei or from Escherichia coli actively catalyze the blunt-end ligation of DNA. This is in contrast to the lack of activity on such substrates by these enzymes under conventional...
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| Publicado en: | Proc Natl Acad Sci U S A |
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| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
National Academy of Sciences
1983
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390173/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6351067/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.19.5852 |
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