Resting of Cryopreserved PBMC Does Not Generally Benefit the Performance of Antigen-Specific T Cell ELISPOT Assays

T cell monitoring is increasingly performed using cryopreserved PBMC. It has been suggested that resting of PBMC after thawing, that is, culturing them overnight in test medium, produces higher antigen-induced spot counts in ELISPOT assays. To evaluate the importance of overnight resting, we systema...

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Bibliografiske detaljer
Main Authors: Kuerten, Stefanie, Batoulis, Helena, Recks, Mascha S., Karacsony, Edith, Zhang, Wenji, Subbramanian, Ramu A., Lehmann, Paul V.
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2012
Fag:
Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3901103/
https://ncbi.nlm.nih.gov/pubmed/24710483
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/cells1030409
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