Use of gene fusions and protein-protein interaction in the isolation of a biologically active regulatory protein: the replication initiator protein of plasmid R6K.

The initiation of DNA replication of plasmid R6K is triggered by a 35-kilodalton initiator protein. The initiator protein had been elusive because of its lability and the lack of a convenient assay procedure to aid its purification. Using recombinant DNA techniques, we have fused the cistron of the...

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Hauptverfasser: Germino, J, Gray, J G, Charbonneau, H, Vanaman, T, Bastia, D
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1983
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC390083/
https://ncbi.nlm.nih.gov/pubmed/6316329
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