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Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA X Fe(II).

In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA X Fe(II) [P5E X Fe(II)] at 0.5 microM cleaves pBR322 plasmid DNA (50 microM in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel electrophoresis. High-resolution denaturing...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Schultz, P G, Dervan, P B
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1983
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC390080/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6417654/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.22.6834
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