Sequence-specific double-strand cleavage of DNA by penta-N-methylpyrrolecarboxamide-EDTA X Fe(II).

In the presence of O2 and 5 mM dithiothreitol, penta-N-methylpyrrolecarboxamide-EDTA X Fe(II) [P5E X Fe(II)] at 0.5 microM cleaves pBR322 plasmid DNA (50 microM in base pairs) on opposite strands to afford discrete DNA fragments as analyzed by agarose gel electrophoresis. High-resolution denaturing...

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Main Authors: Schultz, P G, Dervan, P B
格式: Artigo
語言:Inglês
出版: 1983
主題:
Research Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC390080/
https://ncbi.nlm.nih.gov/pubmed/6417654
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