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Isolation by Covalent Affinity Chromatography of the Penicillin-Binding Components from Membranes of Bacillus subtilis

An affinity chromatography technique was developed to isolate the five penicillin-binding components present in Bacillus subtilis membranes. The proteins were solubilized by the detergent Nonidet P-40, bound covalently to penicillin-substituted Sepharose, and subsequently eluted from the matrix with...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Blumberg, Peter M., Strominger, Jack L.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1972
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389864/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4630162/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.12.3751
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