Isolation by Covalent Affinity Chromatography of the Penicillin-Binding Components from Membranes of Bacillus subtilis
An affinity chromatography technique was developed to isolate the five penicillin-binding components present in Bacillus subtilis membranes. The proteins were solubilized by the detergent Nonidet P-40, bound covalently to penicillin-substituted Sepharose, and subsequently eluted from the matrix with...
Spremljeno u:
| Izdano u: | Proc Natl Acad Sci U S A |
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| Glavni autori: | , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1972
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389864/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4630162/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.12.3751 |
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