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Purification of Insulin-Specific Protease by Affinity Chromatography

A single enzyme that proteolytically degrades insulin was isolated from rat skeletal muscle. This enzyme was purified 1000-fold by a series of steps, including affinity chromatography on insulin bound to agarose at the NH(2)-terminal phenylalanine of the B chain. Insulin linked to agarose at the B-2...

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Detalhes bibliográficos
Main Authors: Duckworth, William C., Heinemann, Martha A., Kitabchi, Abbas E.
Formato: Artigo
Idioma:Inglês
Publicado em: 1972
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC389852/
https://ncbi.nlm.nih.gov/pubmed/4509332
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