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Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells

Viable isolated exocrine cells have been obtained from guinea-pig pancreas by a tissue dissociation procedure using crude collagenase (EC 3.4.4.19) and hyaluronidase (EC 3.2.1.35), chelation of divalent cations, and mild shearing forces. Cell yields are 50-60%, based on recovered DNA, and about 90%...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Amsterdam, A., Jamieson, J. D.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1972
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389700/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4342974/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.69.10.3028
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