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Structural and Functional Characterization of Isolated Pancreatic Exocrine Cells

Viable isolated exocrine cells have been obtained from guinea-pig pancreas by a tissue dissociation procedure using crude collagenase (EC 3.4.4.19) and hyaluronidase (EC 3.2.1.35), chelation of divalent cations, and mild shearing forces. Cell yields are 50-60%, based on recovered DNA, and about 90%...

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Main Authors: Amsterdam, A., Jamieson, J. D.
格式: Artigo
語言:Inglês
出版: 1972
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC389700/
https://ncbi.nlm.nih.gov/pubmed/4342974
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