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Dosage and Cell Line Dependent Inhibitory Effect of bFGF Supplement in Human Pluripotent Stem Cell Culture on Inactivated Human Mesenchymal Stem Cells

Many different culture systems have been developed for expanding human pluripotent stem cells (hESCs and hiPSCs). In general, 4–10 ng/ml of bFGF is supplemented in culture media in feeder-dependent systems regardless of feeder cell types, whereas in feeder-free systems, up to 100 ng/ml of bFGF is re...

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שמור ב:
מידע ביבליוגרפי
Main Authors: Quang, Tara, Marquez, Maribel, Blanco, Giselle, Zhao, Yuanxiang
פורמט: Artigo
שפה:Inglês
יצא לאור: Public Library of Science 2014
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3895015/
https://ncbi.nlm.nih.gov/pubmed/24465853
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0086031
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