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Rapid Exchange of Subunits between Free Ribosomes in Extracts of Escherichia coli

The runoff of a mixture of “heavy” and “light” polysomes has been reported to yield hybrid ribosomes, of intermediate density. However, we found that the dissociation of free heavy ribosomes as they advance in a sucrose gradient causes a serious artefact in the demonstration of hybrid ribosomes. Thi...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Subramanian, Alap R., Davis, Bernard D.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1971
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC389442/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4944627/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.68.10.2453
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