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Pyrosequencing for the rapid and efficient quantification of allele-specific expression
We have developed a rapid and sensitive quantitative assay for the measurement of individual allelic ratios. This assay minimizes time and labor, the need for special restriction endonuclease enzymes for polymorphic sites, and avoids heteroduplex formation seen with traditional quantitative PCR-base...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Landes Bioscience
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3891684/ https://ncbi.nlm.nih.gov/pubmed/23973940 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/epi.25892 |
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