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Action of nicking-closing enzyme on supercoiled and nonsupercoiled closed circular DNA: formation of a Boltzmann distribution of topological isomers.

Highly purified nicking-closing enzyme from mouse cells in 20-fold enzyme/substrate excess converts closed circular native PM2, ColE1, and Minicol DNA into limit product sets of DNAs. Each set has a mean degree of supercoiling of approximately zero. The individual species in the sets differ by delta...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Pulleyblank, D E, Shure, M, Tang, D, Vinograd, J, Vosberg, H P
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1975
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388704/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1060106/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.72.11.4280
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