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Chemical Synthesis of a Primer and Its Use in the Sequence Analysis of the Lysozyme Gene of Bacteriophage T4

We have developed a general approach for determining the nucleotide sequence of a gene, with the aid of a deoxyribonucleotide primer of defined sequence. The selection of the primer sequence was based on a short segment of mRNA sequence of T4 phage lysozyme. A tetradecadeoxyribonucleotide primer was...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Padmanabhan, R., Jay, Ernest, Wu, Ray
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1974
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388489/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4526223/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.6.2510
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