Dual-color-emitting green fluorescent protein from the sea cactus Cavernularia obesa and its use as a pH indicator for fluorescence microscopy

We isolated and characterized a green fluorescent protein (GFP) from the sea cactus Cavernularia obesa. This GFP exists as a dimer and has absorption maxima at 388 and 498 nm. Excitation at 388 nm leads to blue fluorescence (456 nm maximum) at pH 5 and below, and green fluorescence (507 nm maximum)...

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Bibliographic Details
Main Authors: Ogoh, Katsunori, Kinebuchi, Takashi, Murai, Mariko, Takahashi, Takeo, Ohmiya, Yoshihiro, Suzuki, Hirobumi
Format: Artigo
Language:Inglês
Published: John Wiley & Sons, Ltd 2013
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Research Articles
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3884763/
https://ncbi.nlm.nih.gov/pubmed/23468077
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bio.2497
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