Antigen-Secreting Cells: Enumeration of Immunoglobulin-Allotype-Secreting Cells in Nonimmunized Rabbits by Means of Hybrid-Antibody-Coated Erythrocytes in a Reverse Hemolytic Plaque Assay

A reverse hemolytic plaque-forming cell (PFC) assay for the enumeration of immunoglobulin-(Ig)secreting cells in nonimmunized rabbits was developed by using erythrocytes coated with anti-Ig antibody. These indicator cells lysed after reacting with the secreted Ig antigen and complement. The anti-Ig...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Molinaro, Giuseppe A., Maron, Elchanan, Dray, Sheldon
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1974
Soggetti:	Biological Sciences: Immunology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388198/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4133847/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.4.1229
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