Buccal cells DNA extraction to obtain high quality human genomic DNA suitable for polymorphism genotyping by PCR-RFLP and Real-Time PCR

OBJECTIVE: The aim of this study was to evaluate, by PCR-RFLP and Real-time PCR, the yield and quality of genomic DNA collected from buccal cells by mouthwash after different storage times at room temperature. MATERIAL AND METHODS: A group of volunteers was recruited to collect buccal cells using a...

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Detalhes bibliográficos
Main Authors: KÜCHLER, Erika Calvano, TANNURE, Patricia Nivoloni, FALAGAN-LOTSCH, Priscila, LOPES, Taliria Silva, GRANJEIRO, Jose Mauro, AMORIM, Lidia Maria Fonte
Formato: Artigo
Idioma:Inglês
Publicado em: Faculdade de Odontologia de Bauru da Universidade de São Paulo 2012
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Original Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3881822/
https://ncbi.nlm.nih.gov/pubmed/23032210
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1590/S1678-77572012000400013
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