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Structure of Nascent ϕX174 Replicative Form: Evidence for Discontinuous DNA Replication

ϕX174 RF (replicative form) II DNA, labeled in vivo with [methyl-(3)H]thymidine, was isolated from Escherichia coli polA (DNA polymerase I-deficient) and polA(+) cells during RF replication. [(32)P]dCMP was incorporated into the gaps present in the RF II DNA with [α-(32)P]dCTP and T4 DNA polymerase....

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Bibliographic Details
Main Authors: Eisenberg, Shlomo, Denhardt, David T.
Format: Artigo
Language:Inglês
Published: 1974
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC388142/
https://ncbi.nlm.nih.gov/pubmed/4522806
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