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Photochemical Attachment of lac Repressor to Bromodeoxyuridine-Substituted lac Operator by Ultraviolet Radiation

The transducing phage λh80dlac carries the lac operator, whereas wild-type λh80 does not. We find that in high salt (0.18 M KCl), ultraviolet radiation causes the formation of a very stable complex between repressor and 5-bromodeoxyuridine (BrdU)-substituted λh80dlac but not to BrdU-λh80 DNA. Studie...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Lin, Syr-Yaung, Riggs, Arthur D.
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1974
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Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC388134/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4522804/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.71.3.947
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