Reconstitution of a Calcium Pump Using Defined Membrane Components

A (Mg(2+) + Ca(2+))ATPase (ATP phosphohydrolase, EC 3.6.1.3) has been purified from sarcoplasmic reticulum using a single step centrifugation procedure. The preparation is >95% pure by weight and contains only 25-30% of the lipid associated with the enzyme in native sarcoplasmic reticulum. The pu...

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Main Authors: Warren, G. B., Toon, Penelope A., Birdsall, N. J. M., Lee, A. G., Metcalfe, J. C.
Format: Artigo
Sprog:Inglês
Udgivet: 1974
Fag:
Biological Sciences: Biochemistry
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC388063/
https://ncbi.nlm.nih.gov/pubmed/4274560
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