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Formation In Vitro of Infective Joint Molecules of λ DNA by T4 Gene-32 Protein

Half molecules of λ DNA that had been partially digested by λ exonuclease to expose homologous single strands were rejoined by the action of T4 gene-32 protein at 37° in the presence of Mg(++). Measurements of infectivity in su(-) spheroplasts and sedimentation in sucrose demonstrated the formation...

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Detalhes bibliográficos
Main Authors: Wackernagel, Wilfried, Radding, Charles M.
Formato: Artigo
Idioma:Inglês
Publicado em: 1974
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC388020/
https://ncbi.nlm.nih.gov/pubmed/4521814
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