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In vitro self-assembly of human pericyte-supported endothelial microvessels in three-dimensional co-culture: a simple model for interrogating endothelial:pericyte interactions

We describe a method for co-culture of macro or microvascular human endothelial cells (ECs) and pericytes (PCs) within a 3-dimensional (3-D) protein matrix resulting in lumenized EC cords invested by PCs. To prevent apoptotic cell death of ECs in 3-D culture, human umbilical vein or dermal microvasc...

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Detalhes bibliográficos
Main Authors: Waters, JP, Kluger, MS, Graham, M, Chang, WG, Bradley, JR, Pober, JS
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3879598/
https://ncbi.nlm.nih.gov/pubmed/23860328
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1159/000353303
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