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Blind spots of quantitative RNA-seq: the limits for assessing abundance, differential expression, and isoform switching

BACKGROUND: RNA-seq is now widely used to quantitatively assess gene expression, expression differences and isoform switching, and promises to deliver results for the entire transcriptome. However, whether the transcriptional state of a gene can be captured accurately depends critically on library p...

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Detalhes bibliográficos
Main Authors: Rehrauer, Hubert, Opitz, Lennart, Tan, Ge, Sieverling, Lina, Schlapbach, Ralph
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3879183/
https://ncbi.nlm.nih.gov/pubmed/24365034
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-14-370
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