Nested PCR Assay for Detection of Leishmania donovani in Slit Aspirates from Post-Kala-Azar Dermal Leishmaniasis Lesions

A nested PCR assay to detect parasite DNA in slit aspirates from skin lesions of patients with post-kala-azar dermal lesihmaniasis (PKDL) is described. PCR results were positive in 27 of 29 (93%) samples by nested PCR assay, while only 20 of 29 (69%) were positive in a primary PCR assay. The nested...

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Hlavní autoři: Sreenivas, Gannavaram, Ansari, N. A., Kataria, Joginder, Salotra, Poonam
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology 2004
Témata:
Parasitology
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC387583/
https://ncbi.nlm.nih.gov/pubmed/15071047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.4.1777-1778.2004
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