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Overview of the most prevalent hypothalamus-specific mRNAs, as identified by directional tag PCR subtraction.

We applied the directional tag PCR subtractive hybridization method to construct a rat hypothalamic cDNA library from which cerebellar and hippocampal sequences had been depleted, enriching 20-30-fold for sequences expressed selectively in the hypothalamus. We studied a sample of 94 clones selected...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Principais autores: Gautvik, K M, de Lecea, L, Gautvik, V T, Danielson, P E, Tranque, P, Dopazo, A, Bloom, F E, Sutcliffe, J G
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1996
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC38742/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8710940/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.93.16.8733
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