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Transition-state stabilization in Escherichia coli ribonuclease P RNA-mediated cleavage of model substrates

We have used model substrates carrying modified nucleotides at the site immediately 5′ of the canonical RNase P cleavage site, the −1 position, to study Escherichia coli RNase P RNA-mediated cleavage. We show that the nucleobase at −1 is not essential but its presence and identity contribute to effi...

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Bibliografske podrobnosti
Main Authors: Wu, Shiying, Chen, Yu, Mao, Guanzhong, Trobro, Stefan, Kwiatkowski, Marek, Kirsebom, Leif A.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2014
Teme:
RNA
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3874170/
https://ncbi.nlm.nih.gov/pubmed/24097434
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkt853
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