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Generation of single and double knockdowns in polarized epithelial cells by retrovirus-mediated RNA interference

RNA interference (RNAi) is a ubiquitous mechanism of eukaryotic gene regulation that can be exploited for specific gene silencing. Retroviruses have been successfully used for stable expression of short hairpin RNAs in mammalian cells, leading to persistent inhibition of gene expression by RNAi. Her...

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Detalhes bibliográficos
Main Authors: Schuck, Sebastian, Manninen, Aki, Honsho, Masanori, Füllekrug, Joachim, Simons, Kai
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC387348/
https://ncbi.nlm.nih.gov/pubmed/15051873
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401285101
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