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Generation of single and double knockdowns in polarized epithelial cells by retrovirus-mediated RNA interference
RNA interference (RNAi) is a ubiquitous mechanism of eukaryotic gene regulation that can be exploited for specific gene silencing. Retroviruses have been successfully used for stable expression of short hairpin RNAs in mammalian cells, leading to persistent inhibition of gene expression by RNAi. Her...
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Main Authors: | , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
National Academy of Sciences
2004
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC387348/ https://ncbi.nlm.nih.gov/pubmed/15051873 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0401285101 |
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