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Isolation of yeast mutants defective in protein targeting to the vacuole.

We have constructed a PRC1-SUC2 gene fusion that directs the synthesis in Saccharomyces cerevisiae of a hybrid polypeptide consisting of a 433-residue amino-terminal domain derived from the yeast vacuolar protease carboxypeptidase Y (CPY; EC 3.4.16.1) and a 511-residue carboxyl-terminal domain deriv...

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Main Authors: Bankaitis, V A, Johnson, L M, Emr, S D
Formato: Artigo
Idioma:Inglês
Publicado: 1986
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC387077/
https://ncbi.nlm.nih.gov/pubmed/3538017
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