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Functional Characterization of the Origin of Replication of pRN1 from Sulfolobus islandicus REN1H1

Plasmid pRN1 from Sulfolobus islandicus REN1H1 is believed to replicate by a rolling circle mechanism but its origin and mechanism of replication are not well understood. We sought to create minimal expression vectors based on pRN1 that would be useful for heterologous gene expression in S. acidocal...

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Autori principali: Joshua, Chijioke J., Perez, Luis D., Keasling, Jay D.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3869888/
https://ncbi.nlm.nih.gov/pubmed/24376833
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0084664
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