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Photocrosslinking of the signal sequence of nascent preprolactin to the 54-kilodalton polypeptide of the signal recognition particle.

Photoreactive moieties were incorporated into nascent polypeptides in a wheat germ protein-synthesizing system by using a plasmid-derived preprolactin mRNA and a Lys-tRNA analog, N epsilon-(5-azido-2-nitrobenzoyl)-Lys-tRNA (epsilon ANB-Lys-tRNA). The presence of the abnormally large amino acid side...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Krieg, U C, Walter, P, Johnson, A E
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1986
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC386979/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3095839/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.22.8604
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