Translation of a synthetic two-cistron mRNA in Escherichia coli.
A synthetic two-cistron expression system was constructed for the high-level expression of eukaryotic genes in Escherichia coli. This system was designed to overcome translational inhibition of mRNAs containing eukaryotic sequences. The first cistron in this system is a 31-base A + T-rich synthetic...
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| Pubblicato in: | Proc Natl Acad Sci U S A |
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| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1986
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC386959/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3534891/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.22.8506 |
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