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Use of monoclonal antibodies in the purification and characterization of 1-aminocyclopropane-1-carboxylate synthase, an enzyme in ethylene biosynthesis

1-Aminocyclopropane-1-carboxylate (ACC) synthase (EC 4.4.1.14), extracted from tomato pericarp tissue, was purified 6500-fold by conventional and high-performance liquid chromatography. Two-dimensional gel electrophoresis of this preparation indicated that ACC synthase activity was associated with a...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Bleecker, Anthony B., Kenyon, William H., Somerville, Shauna C., Kende, Hans
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1986
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC386800/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/16593770/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.20.7755
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