Analysis of High Affinity Self-Association by Fluorescence Optical Sedimentation Velocity Analytical Ultracentrifugation of Labeled Proteins: Opportunities and Limitations

Sedimentation velocity analytical ultracentrifugation (SV) is a powerful first-principle technique for the study of protein interactions, and allows a rigorous characterization of binding stoichiometry and affinities. A recently introduced commercial fluorescence optical detection system (FDS) permi...

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Autori principali: Zhao, Huaying, Lomash, Suvendu, Glasser, Carla, Mayer, Mark L., Schuck, Peter
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2013
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3866193/
https://ncbi.nlm.nih.gov/pubmed/24358283
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0083439
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