Affinity purification of sequence-specific DNA binding proteins.

We describe a method for affinity purification of sequence-specific DNA binding proteins that is fast and effective. Complementary chemically synthesized oligodeoxynucleotides that contain a recognition site for a sequence-specific DNA binding protein are annealed and ligated to give oligomers. This...

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Veröffentlicht in:	Proc Natl Acad Sci U S A
Hauptverfasser:	Kadonaga, J T, Tjian, R
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	National Academy of Sciences 1986
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC386402/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3461465/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.83.16.5889
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