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Folding of 16S RNA in a signal-producing structure for the detection of bacteria

[Image: see text] Sixty four DNA strands hybridize to 16S rRNA to form 32 deoxyribozyme catalytic cores that produce fluorescent signal. The approach allows detection of 0.6 pM 16S rRNA, or ~ 3×10(4) bacterial cells in a PCR-free format.

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Autors principals: Gerasimova, Yulia V., Kolpashchikov, Dmitry M.
Format: Artigo
Idioma:Inglês
Publicat: 2013
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3863991/
https://ncbi.nlm.nih.gov/pubmed/24038733
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/anie.201303919
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