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Systematic reconstruction of RNA functional motifs with high throughput microfluidics

We present RNA-MITOMI, a microfluidic platform for integrated synthesis and functional assays across thousands of RNAs. The interaction of a comprehensive library of RNA mutants with stem loop binding protein precisely defined the RNA structural and sequence features that govern affinity. The functi...

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Detalhes bibliográficos
Main Authors: Martin, Lance, Meier, Matthias, Lyons, Shawn M., Sit, Rene V., Marzluff, William F., Quake, Stephen R., Chang, Howard Y.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3863600/
https://ncbi.nlm.nih.gov/pubmed/23142872
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.2225
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