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Construction and identification of the recombinant plasmid pET30a-EgA31-Eg95 of Echinococcus granulosus
To clone the Eg95 and EgA31 antigen genes into the prokaryotic expression plasmid pET30a-EgA31-Eg95, we expressed the recombinant protein EgA31-Eg95 and confirmed with western blot analysis. The total RNA was extracted from the protoscoleces of Echinococcus granulosus (E. granulosus) adult worms. Th...
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| Main Authors: | , , , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
D.A. Spandidos
2014
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3861358/ https://ncbi.nlm.nih.gov/pubmed/24348791 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3892/etm.2013.1393 |
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