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Allele-specific qRT-PCR demonstrates superior detection of single nucleotide polymorphisms as genetic markers for West Nile virus compared to Luminex® and quantitative sequencing
To enable in vivo and in vitro competitive fitness comparisons among West Nile viruses (WNV), three reference viruses were genetically marked by site-directed mutagenesis with five synonymous nucleotide substitutions in the envelope gene region of the genome. Phenotypic neutrality of the mutants was...
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| Auteurs principaux: | , , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2013
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3856659/ https://ncbi.nlm.nih.gov/pubmed/24121135 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2013.09.014 |
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