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Continuous High-resolution Microscopic Observation of Replicative Aging in Budding Yeast
We demonstrate the use of a simple microfluidic setup, in which single budding yeast cells can be tracked throughout their entire lifespan. The microfluidic chip exploits the size difference between mother and daughter cells using an array of micropads. Upon loading, cells are trapped underneath the...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
MyJove Corporation
2013
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3855928/ https://ncbi.nlm.nih.gov/pubmed/23995364 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50143 |
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