Deciphering Ligand Specificity of a Clostridium thermocellum Family 35 Carbohydrate Binding Module (CtCBM35) for Gluco- and Galacto- Substituted Mannans and Its Calcium Induced Stability

This study investigated the role of CBM35 from Clostridium thermocellum (CtCBM35) in polysaccharide recognition. CtCBM35 was cloned into pET28a (+) vector with an engineered His(6) tag and expressed in Escherichia coli BL21 (DE3) cells. A homogenous 15 kDa protein was purified by immobilized metal i...

Descrizione completa

Salvato in:
Dettagli Bibliografici
Autori principali: Ghosh, Arabinda, Luís, Ana Sofia, Brás, Joana L. A., Pathaw, Neeta, Chrungoo, Nikhil K., Fontes, Carlos M. G. A., Goyal, Arun
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2013
Soggetti:
Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3855759/
https://ncbi.nlm.nih.gov/pubmed/24324599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0080415
Tags: Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne! !

Documenti analoghi