Deciphering Ligand Specificity of a Clostridium thermocellum Family 35 Carbohydrate Binding Module (CtCBM35) for Gluco- and Galacto- Substituted Mannans and Its Calcium Induced Stability

This study investigated the role of CBM35 from Clostridium thermocellum (CtCBM35) in polysaccharide recognition. CtCBM35 was cloned into pET28a (+) vector with an engineered His(6) tag and expressed in Escherichia coli BL21 (DE3) cells. A homogenous 15 kDa protein was purified by immobilized metal i...

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Detalhes bibliográficos
Main Authors: Ghosh, Arabinda, Luís, Ana Sofia, Brás, Joana L. A., Pathaw, Neeta, Chrungoo, Nikhil K., Fontes, Carlos M. G. A., Goyal, Arun
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3855759/
https://ncbi.nlm.nih.gov/pubmed/24324599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0080415
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