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Methanol fixation of plant tissue for Scanning Electron Microscopy improves preservation of tissue morphology and dimensions

BACKGROUND: It is well known that preparation of biological (plant and animal) tissues for Scanning Electron Microscopy (SEM) by chemical fixation and critical point drying results in shrinkage of tissues, often by up to 20-30%, depending on the tissue type and fixation protocol used. We sought to i...

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Detalhes bibliográficos
Main Authors: Talbot, Mark J, White, Rosemary G
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3853006/
https://ncbi.nlm.nih.gov/pubmed/24083940
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1746-4811-9-36
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