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A Simplified Approach for Oocyte Enucleation in Mammalian Cloning

Despite its success in almost all farm and laboratory animals, somatic cell nuclear transfer (SCNT) is still a low-efficiency technique. In this investigation, we determined the impact of each enucleation step on oocyte viability (assessed by parthenogenetic activation): Hoechst (HO) staining, cytoc...

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Detalhes bibliográficos
Main Authors: Iuso, Domenico, Czernik, Marta, Zacchini, Federica, Ptak, Grazyna, Loi, Pasqualino
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc. 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3848443/
https://ncbi.nlm.nih.gov/pubmed/24219576
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/cell.2013.0051
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