Cloning and Analysis of a Large Plasmid pBMB165 from Bacillus thuringiensis Revealed a Novel Plasmid Organization

In this study, we report a rapid cloning strategy for large native plasmids via a contig linkage map by BAC libraries. Using this method, we cloned a large plasmid pBMB165 from Bacillus thuringiensis serovar tenebrionis strain YBT-1765. Complete sequencing showed that pBMB165 is 77,627 bp long with...

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Bibliografiske detaljer
Main Authors: Wang, Yueying, Peng, Donghai, Dong, Zhaoxia, Zhu, Lei, Guo, Suxia, Sun, Ming
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2013
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3847046/
https://ncbi.nlm.nih.gov/pubmed/24312580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0081746
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