Recombinant human lecithin-cholesterol acyltransferase Fc fusion: Analysis of N- and O-linked glycans and identification and elimination of a xylose-based O-linked tetrasaccharide core in the linker region

Recombinant human lecithin-cholesterol acyltransferase Fc fusion (huLCAT-Fc) is a chimeric protein produced by fusing human Fc to the C-terminus of the human enzyme via a linker sequence. The huLCAT-Fc homodimer contains five N-linked glycosylation sites per monomer. The heterogeneity and site-speci...

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Main Authors: Spahr, Chris, Kim, Justin J, Deng, Sihong, Kodama, Paul, Xia, Zhen, Tang, Jay, Zhang, Richard, Siu, Sophia, Nuanmanee, Noi, Estes, Bram, Stevens, Jennitte, Zhou, Mingyue, Lu, Hsieng S
Formato: Artigo
Idioma:Inglês
Publicado em: Blackwell Publishing Ltd 2013
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Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3843628/
https://ncbi.nlm.nih.gov/pubmed/24115046
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2373
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