An integrated strategy for efficient vector construction and multi-gene expression in Plasmodium falciparum

BACKGROUND: The construction of plasmid vectors for transgene expression in the malaria parasite, Plasmodium falciparum, presents major technical hurdles. Traditional molecular cloning by restriction and ligation often yields deletions and re-arrangements when assembling low-complexity (A + T)-rich...

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Bibliographic Details
Main Authors: Wagner, Jeffrey C, Goldfless, Stephen J, Ganesan, Suresh M, Lee, Marcus CS, Fidock, David A, Niles, Jacquin C
Format: Artigo
Language:Inglês
Published: BioMed Central 2013
Subjects:
Methodology
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3842810/
https://ncbi.nlm.nih.gov/pubmed/24160265
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2875-12-373
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