Selection of functional cDNAs by complementation in yeast.

Yeast cDNA was prepared in a yeast expression plasmid to generate a cDNA plasmid pool composed of approximately 40,000 members. Several yeast mutants were transformed with the cDNA plasmid pool, and the cDNAs for ADC1, HIS3, URA3, and ASP5 were isolated by functional complementation. Restriction enz...

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Detaylı Bibliyografya
Asıl Yazarlar: McKnight, G L, McConaughy, B L
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 1983
Konular:
Research Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC384048/
https://ncbi.nlm.nih.gov/pubmed/6192432
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