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In vitro synthesis of bacteriophage phi X174 by purified components.

An in vitro system capable of synthesizing infectious phi X174 phage particles was reconstituted from purified components. The synthesis required phi X174 supercoiled replicative form DNA, phi X174-encoded proteins A, C, J, and prohead, Escherichia coli DNA polymerase III holoenzyme, rep protein, an...

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Pubblicato in:Proc Natl Acad Sci U S A
Autori principali: Aoyama, A, Hamatake, R K, Hayashi, M
Natura: Artigo
Lingua:Inglês
Pubblicazione: National Academy of Sciences 1983
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Accesso online:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC384003/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/6224217/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.80.14.4195
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