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Reconstitution of RNase P activity from inactive RNA and protein.

RNase P preparations from Escherichia coli can be separated into RNA and protein by chromatography, in buffers containing 7 M urea, on Sephadex G-200, DEAE-Sephadex, or CM-Sephadex columns. Neither RNA nor protein components alone exhibits any RNase activity. RNase P activity can be reconstituted by...

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Detalles Bibliográficos
Main Authors: Kole, R, Altman, S
Formato: Artigo
Idioma:Inglês
Publicado: 1979
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC383921/
https://ncbi.nlm.nih.gov/pubmed/386349
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