Molecular cloning and partial characterization of delta-crystallin cDNA sequences in a bacterial plasmid.

Double-stranded cDNA synthesized from delta-crystallin mRNA isolated from lens fiber cells of 15-day-old embryonic chicken was cloned in Escherichia coli chi 1776 in the Pst I site of the plasmid pBR322 by using the oligo(dC) . oligo(dG) joining procedure. Twelve Amps Tetr transformants contained se...

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Bibliographic Details
Main Authors: Bhat, S P, Piatigorsky, J
Format: Artigo
Language:Inglês
Published: 1979
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC383812/
https://ncbi.nlm.nih.gov/pubmed/386337
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