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Structure-independent nucleotide sequence analysis.
Substitution of inosine for granosine in the nucleic acid fragments synthesized for the sequencing of RNA effectively prevents the formation of secondary structures during electrophoretic analysis. Consequently, the mobility of each fragment in the sequencing gel is a strict function of its molecula...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
1979
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC383572/ https://ncbi.nlm.nih.gov/pubmed/287063 |
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