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Positive control of lac operon expression in vitro by guanosine 5′-diphosphate 3′-diphosphate

Maximal expression of the Escherichia coli lactose operon in a coupled in vitro transcription-translation system from a Salmonella typhimurium relA mutant was strongly dependent upon addition of guanosine 5′-diphosphate 3′-diphosphate (ppGpp). Without added ppGpp, at saturating 3′,5′-cyclic AMP (cAM...

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Bibliographic Details
Main Authors: Primakoff, Paul, Artz, Stanley W.
Format: Artigo
Language:Inglês
Published: 1979
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC383463/
https://ncbi.nlm.nih.gov/pubmed/109832
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