Efficient cellular fractionation improves RNA sequencing analysis of mature and nascent transcripts from human tissues

BACKGROUND: The starting material for RNA sequencing (RNA-seq) studies is usually total RNA or polyA+ RNA. Both forms of RNA represent heterogeneous pools of RNA molecules at different levels of maturation and processing. Such heterogeneity, in addition to the biases associated with polyA+ purificat...

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Main Authors: Zaghlool, Ammar, Ameur, Adam, Nyberg, Linnea, Halvardson, Jonatan, Grabherr, Manfred, Cavelier, Lucia, Feuk, Lars
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2013
Teme:
Research Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3833653/
https://ncbi.nlm.nih.gov/pubmed/24225116
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-13-99
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